Aggregation Stability of a Monoclonal Antibody During Downstream Processing

Development of a Novel Inhibitory Chimeric Anti-HER2 Monoclonal Antibody

A monoclonal antibody against human thrombospondin inhibits platelet aggregation.

A monoclonal antibody (C6.7) has been generated against the calcium-replete form of human platelet thrombospondin (TSP). C6.7 is specific for TSP as determined by both competitive radioimmunoassay and immunoprecipitation. This antibody inhibits both thrombin- and A23187-induced aggregation of gel-filtered platelets in a concentration-dependent manner without affecting the secretion of serotonin...

Multimodal chromatography: debottlenecking the downstream processing of monoclonal antibodies

2015 The commercial potential of monoclonal antibodies (mAbs) has been continuously increasing during the last years alongside with the number of approved mAb-based drugs and clinical trials. Despite their effectiveness and safety, the general access to this class of biopharmaceuticals is barred by high selling prices. Downstream processing is now considered the bottleneck in the manufacturing ...

Downstream Processing of Monoclonal Antibodies: Current Practices and Future Opportunities

s of Papers, 232nd ACS Meeting, San Francisco, CA, 2006. Yigzaw, Y. , Piper, R. , Tran , M. , Shukla , A.A. ( 2006 ). Exploitation of the adsorptive properties of depth filters for host cell protein removal during monoclonal antibody purifi cation. Biotechnology Progress 22, 288–296. Roque, A.C.A. , Silva, C.S.O. , Taipa, M.A. (2007). Affi nit...

A Capture ELISA for the Diagnosis of Visceral Leishmaniasis Using a Monoclonal Antibody against a Leishmanial

capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...